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Single Step Guaranteed Knockdown No transfection reagents needed Ideal for cellular and animal studies
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AUMantagomir

miRNA-Inhibition

Find the right RNA silencing
product for you in 3 simple steps

No toxicity. No transfection reagents needed.

AUMantagomir-fig-1

AUMantagomir provides potent miRNA inhibition by using using FANA ASO technology, which is a third generation chemical modification platform. FANA ASO technology allows simple and efficient delivery into difficult-to-transfect cells including primary cells. Additionally, FANA ASOs can also be used for animal and preclinical studies without the need of transfection reagents or additional chemical formulations.

Self-delivering FANA ASOs work by binding to the target miRNA and prevent hybridization with their mRNA targets (Figure 1). FANA ASOs can also enter the nucleus and can be used to target both cytoplasmic and nuclear miRNA.

Lipid-based transfection and electroporation are widely utilized, conventional methods to deliver siRNA into the cells. However, in many primary cells, particularly immune cells, hematopoietic cells and neurons, lipid reagents and electroporation are associated with high toxicity and poor transfection efficiency. Alternative delivery methods, such as viral vectors, require laborious optimization and viral production steps, and carry associated risk of genome integration.

FANA ASOs are uniquely designed and manufactured using 2′-deoxy-2′- fluoro-β-d-arabinonucleic acid (FANA) chemical modifications that enhance intracellular stability of the oligos, providing very high specificity as well as high binding affinity to the target mRNA. The FANA modifications also allow for the oligos to be self-delivered into cells without any transfection reagents or electroporation. FANA ASOs can be used for animal studies without the need of delivery formulations or conjugates.

Case Studies

Efficient knockdown of miR-134 by FANAs in treated rat hippocampal slices

RT-qPCR analysis showed a decrease in miR-134 expression in slices treated with FANA-miR-134 compared to scrambled control. By using FANA ASOs the role of miR-134 in Alzheimer’s disease was elucidated (Figure 2). Adapted from Baby, N. et al. 2019. Aging Cell.

Ordering Information

Name Application Purification Study Model
AUMantagomir miRNA inhibition RPC Cell lines and primary cells
AUMantagomir miRNA inhibition HPLC Sensitive primary cells, animal models, and preclinical studies
AUMantagomir miRNA inhibitionn In vivo Animal models and preclinical studies
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Notes:
  • Concentration: Please review our FANA ASO Calculation Guidelines.

  • Duration of effect: • Duration of effect: Depending on the experiment, different time points can be used to measure inhibition or related effects for up to several days (and weeks in some cases) using a single dose or multiple doses. In certain cases (especially for very fast-growing cells), the knockdown effect may be reduced after a few days. In such cases, simply add more FANA ASOs to the cell culture to maintain knockdown levels.

  • Label: Label: FANA ASOs can be labeled with any fluorescent label (red, green, yellow or others) to monitor cellular uptake or perform other biodistribution or probing experiments in animal studies. Additionally, FANA ASOs can also be labeled with any conventional tags (eg. biotin).
  • Storage: FANA ASOs are shipped in lyophilized form. Upon arrival, store them in -20°C. When ready to use, re-suspend FANA ASOs in sterile water or appropriate buffer at the desired concentration. Aliquot re-suspended FANA ASOs in aliquots to avoid multiple freeze-thaw cycles.
  • Size:FANA ASOs are available in 10, 25, 50, 100, 250, 500 and 1,000 nmoles. Higher amounts are also available upon inquiry.
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