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Single Step Guaranteed Knockdown No transfection reagents needed Ideal for cellular and animal studies
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AUMsaver

RNA-knockdown

One product for your RNA
knockdown and regulation needs

A singular product that can knockdown, regulate, and block at lower price.

AUMantagomir-fig-1

AUMsavercan be used to efficiently silence or regulate different RNA modalities, such as messenger RNA, micro-RNA and long non-coding RNA for in vitro studies. In addition, ASOs can be designed to act as steric blockers without eliciting RNase-H mediated degradation. AUMsaver provides potent knockdown of the target RNA with high sequence specificity.

AUMsaver use RNase H-mediated cleavage (Figure 1), as opposed to the RNAi pathway involving the RISC complex (which is prone to off target effects). RNAse H recognizes the ASO/RNA duplex and cleaves the RNA within the hybrid. Following cleavage, the fragmented RNA is further degraded by nucleases, whereas the ASOs are recycled within the cell.

Tell Us Your Gene
AUMantagomir-fig-1

AUMsavercan be used to efficiently silence or regulate different RNA modalities, such as messenger RNA, micro-RNA and long non-coding RNA for in vitro studies. In addition, ASOs can be designed to act as steric blockers without eliciting RNase-H mediated degradation. AUMsaverprovides potent knockdown of the target RNA with high sequence specificity.

As opposed to the RNAi pathway (involving RISC), single-stranded ASOs use RNase H-mediated cleavage (Figure 1). This mode of lncRNA knockdown is simpler than siRNA-mediated knockdown and eliminates RISC-associated off-target effects often observed with siRNA. The ASOs can enter the nucleus and can be used to target both cytoplasmic and nuclear RNA.

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AUMantagomir-fig-1

AUMsaveran be used to efficiently silence or regulate different RNA modalities, such as messenger RNA, micro-RNA and long non-coding RNA for in vitro studies. In addition, ASOs can be designed to act as steric blockers without eliciting RNase-H mediated degradation. AUMsaverprovides potent knockdown of the target RNA with high sequence specificity.

AUMsaverASOs work by binding to the target miRNA and prevent hybridization with their mRNA targets (Figure 1). ASOs can also enter the nucleus and can be used to target both cytoplasmic and nuclear miRNA.

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Case Studies

QRT-PCR analysis of c-MYB expression at 96 hours in K562 cells

QRT-PCR assay performed on RNA isolated from K562 cells transfected with ASOs and PBS control. Data are presented as a function of c-MYB mRNA relative to GAPDH mRNA (Figure 2). Adapted from Karlota, A. et al. 2006. Nucleic Acids Research.

Knockdown of yylncT lncRNA by AUM’s ASOs in human embryonic stem cells

ASOs mediated depletion of the target transcript led to a significant decrease in early mesoderm markers, suggesting that yylncT is a mediator of the mesoderm fate of human embryonic stem cells (Figure 3). Adapted from Frank, S. et al. 2018. Cell Stem Cell.

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Protocol

Ordering Information

Name Application Purification Study Model
AUMsaver mRNA knockdown RPC Cell lines
AUMsaver lncRNA knockdown RPC Cell lines
AUMsaver miRNA inhibition RPC Cell lines
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Notes:
  • Concentration: Please review our FANA ASO Calculation Guidelines.

  • Duration of effect: Depending on the experiment, different time points can be used to measure inhibition or related effects for up to several days (and weeks in some cases) using a single dose or multiple doses. In certain cases (especially for very fast-growing cells), the knockdown effect may be reduced after a few days. In such cases, simply add more FANA ASOs to the cell culture to maintain knockdown levels.

  • Label: FANA ASOs can be labeled with any fluorescent label (red, green, yellow or others) to monitor cellular uptake or perform other biodistribution or probing experiments in animal studies. Additionally, FANA ASOs can also be labeled with any conventional tags (eg. biotin).
  • Storage:FANA ASOs are shipped in lyophilized form. Upon arrival, store them in -20°C. When ready to use, re-suspend FANA ASOs in sterile water or appropriate buffer at the desired concentration. Aliquot re-suspended FANA ASOs in aliquots to avoid multiple freeze-thaw cycles.
  • Size:FANA ASOs are available in 10, 25, 50, 100, 250, 500 and 1,000 nmoles. Higher amounts are also available upon inquiry.
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